In behaviour that has become the normal for the 45th US President, Trump took to his personal Twitter account to post a late-night tweet bashing James.
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In his interview with Don Lemon, James said that sport had offered him the opportunity to meet people of different backgrounds and race, adding: "Sports has never been something that divides people".
James has said that Trump "doesn't understand the power that he has for being the leader of this handsome country".More news: Buttler looks to draw inspiration from Kohli
"I can't sit back and say nothing", said James, who lists participating in youth athletics as one of the most inclusive experiences of his upbringing.
James said Trump is creating a wedge by using situations like National Football League player Colin Kaepernick kneeling during the anthem and "uninviting" NBA star Steph Curry to the White House.
While James has not commented on Trump's tweet, Lemon defended the former Miami Heat and Cleveland Cavalier star on Saturday, while taking a jab at the president.
Others, including OH governor John Kasich have responded. "Going to White House was a great honor until you showed up!"More news: Turkey 'will retaliate' over U.S. sanctions as crisis grows
The US leader has criticized players who "take a knee" during the playing of the anthem, calling for them to be banned or fired.