The ad will debut on NBC in the NFL's opening round encounter between the Atlanta Falcons and Philadelphia Eagles.
Nike continues to anger fans as it's now doubled down on its Kaepernick partnership and released a new ad narrated by the former San Francisco 49ers player - who is also responsible for starting the controversial national anthem kneeling protests in the NFL.
He later opted to take a knee after Army veteran Nate Boyer told him it would sit better with service members.More news: Lack of train places one in 4 individuals in danger, World Health Organization says
Press play on the video above to see if you side with Trump or Kaepernick.
M Science also found the latest boycott calls are mild in comparison to the outrage sparked a year ago by the NFL's decision to allow players to kneel during the national anthem.
Before Nike's announcement, about half of Americans said they were absolutely certain or very likely to buy Nike products, but that percentage has dropped to 39 percent.
"We believe Colin is one of the most inspirational athletes of this generation, who has leveraged the power of sport to help move the world forward", said Gino Fisanotti, Nike's vice president of brand for North America.More news: Department of Justice Charges North Korean Spy for 2014 Sony Hacking
Colin Kaepernick will make an appearance during the National Football League season opener on Thursday night - although it won't be on the field.
Having been honoured at a fashion show on Tuesday, Nike athlete James said: "I stand with Nike, all day, every day". And then there's President Donald Trump, who predictably tweeted his disapproval. "Even if means sacrificing everything".
On Monday, Nike released a promotional image for their new Kaepernick-inspired campaign which featured a black and white portrait of the player superimposed with the slogan "Believe in something".
"As our country has become divided and name calling and hate has become normalized, we were particularly disheartened by her comment on Facebook yesterday", the petition said in part. However, the decision has been met with intense controversy, with threats of boycotts and even some individuals burning their Nike gear.More news: Active Shooter Reported in Downtown Cincinnati