"He added: "There is nothing special and nothing criminal about it, I" m telling you".
Scotland Yard and the UK's Crown Prosecution Service have said there is enough evidence to charge the men, who are understood to have travelled to London from Moscow on 2 March on Russian passports.
BBC Moscow correspondent Sarah Rainsford called Mr Putin's words a "tantalising hint" that the two men will speak "very soon".
British police released a batch of photos of the the men it identified as Alexander Petrov and Ruslan Boshirov - names that investigators said likely were aliases used for the operation.
"I hope they will turn up themselves and tell about themselves", he said.More news: Forza Horizon 4 is getting a James Bond day one auto pack
For months, British and USA authorities have blamed the Kremlin for the attack, and London said Boshirov and Petrov were Russian intelligence agents.
LONDON - Britain's attempts to get an explanation from Russia over the poisoning of a former Russian spy in Salisbury have been met with obfuscation and lies and that hasn't changed, Prime Minister Theresa May's spokesman said on Wednesday.
"They are civilians, of course", he said, apparently responding to a claim by the British authorities that the two suspects are members of Russia's military intelligence agency. I would like to appeal to them so that they hear us today.
Asked about Putin's remarks, Prime Minister Theresa May's spokesman said Britain's attempts to get an explanation from Russian Federation over the poisoning of the Skripals had always been met with obfuscation and lies and that had not changed.
Putin and Russian Federation have vehemently denied having anything to do with the poisoning cases.More news: U.S. Identifies Remains of Two Service Members Returned From North Korea
"We have repeatedly asked Russian Federation to account for what happened in Salisbury in March and they have replied with obfuscation and lies", Slack said.
Russian President Vladimir Putin attends the plenary session of the Eastern Economic Forum in Vladivostok on September 12, 2018.
The suspects fled to Russian Federation, who won't extradite them, but they would be arrested if they entered any European Union country. "Hours after their final visit to Salisbury, they took a late-night flight back to Moscow on March 4 - the same day the Skripals were sickened". The government exposed the role of the GRU, its operatives and its methods.
Skripal's niece Viktoria, who lives in Russia and often voices pro-Kremlin arguments on Russian television talk shows, told the Interfax news agency Wednesday that she knows "through her own sources" that the men identified as Petrov and Boshirov are "ordinary men" who are "shocked" by the accusations.More news: 6 people are dead after shooting spree in California