Ronaldo's omission comes in the wake of the rape allegations against him by former American model Kathryn Mayorga.
Las Vegas police confirmed on Tuesday they had initially investigated a complaint in June 2009, but added they had no suspect in the case.More news: British PM Theresa May kicks off keynote speech with ABBA's 'Dancing Queen'
The five-time Ballon d'Or victor missed the two internationals Portugal played in September.
Mayorga has been interviewed by law enforcement twice in recent months and will cooperate further with the Las Vegas police department's reopening of its investigation into her case.
It was there that Ronaldo forced himself on Ms. Mayorga while she begged him to stop, the filing states.
Juventus forward and global superstar Cristiano Ronaldo is embroiled in a scandal, and it's reached the point where some of his principal sponsors have been forced to come out and comment.More news: Google Assistant is getting an updated UI for mobile devices
She signed a $375,000 settlement agreement, according to the suit, that required her silence on the matter though her lawyers said it should now be voided.
Portugal coach Fernando Santos said Ronaldo also will not be called up for the next round of worldwide games in November.
Ronaldo transferred to Italian club Juventus this summer from Real Madrid at a reported fee of $123m.
At the moment, the publisher has no plans to replace Ronaldo as the cover star of its hit soccer game, though that could change as the investigation goes on, depending on the results.
While other players might be winding down their careers at 33, Ronaldo is still in demand by leading clubs around the world.More news: Samsung released Galaxy Note 9 in the new color